Phospho-egfr tyr1068
WebA synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human phospho EGFR. Formulation: 1X PBS, 0.09% NaN3, 0.2% BSA: Isotype: Rabbit IgGk: Preparation: Protein A+G: Recommended Usage: For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. WebPhospho-EGFR (Tyr1068) Antibody (PA5-85739) in ICC/IF Immunocytochemistry analysis of Phospho-EGFR (Tyr1068) in 4% paraformaldehyde-fixed A431 cells using Phospho-EGFR (Tyr1068) polyclonal antibody ( Product # PA585739) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody. Product Details Product Specific …
Phospho-egfr tyr1068
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WebThe GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068. A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc … WebEpidermal Growth Factor Receptor antibody (EGFR) is a transmembrane glycoprotein receptor tyrosine kinase and is activated by EGF. The carboxy terminal tyrosine residues on EGFR, Tyr1068, Tyr1148 and Tyr1173 are major sites of autophosphorylation, which occurs as the result of EGF binding. Once activated, phosphotyrosines mediate the binding of …
WebThis assay kit contains antibodies which recognize the phospho -Tyr1068 epitope, and a distal epitope, on epidermal growt h factor receptor (EGFR). The protein detected by this … WebThis assay kit contains antibodies which recognize the phospho -Tyr1068 epitope, and a distal epitope, on epidermal growt h factor receptor (EGFR). The protein detected by this kit correspond s to GenBank Accession NP_005219. EGFR is also known as ERBB, HER1, mENA, ERBB1 and PIG61. These antibodies recognize EGFR of human and mouse origin.
WebNational Center for Biotechnology Information WebAug 6, 2015 · All of the cell lines with known EGFR-activating mutations (HCC827, H1975 and H1650) and the EGFR-WT Calu3 cell line displayed prominent EGFR tyr1068 phosphorylation and were sensitive (<30% ...
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WebBlocking Peptide. The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein with an extracellular ligand-binding domain and a cytoplasmic domain with intrinsic tyrosine kinase activity. The cytoplasmic domain has a C-terminal region with multiple autophosphorylation sites (Tyr-992, 1068, 1086, 1148, and 1173). dickeys lincolnWebApr 12, 2024 · We previously reported that the combined application of Ephedra Herb extract (EHE) and epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor (TKI), erlotinib, is effective in suppressing the growth of erlotinib-resistant non-small-cell lung cancer (NSCLC) cell line, H1993, xenograft tumor, and cell proliferation, and that EHE … citizens cash back card loginWebPhospho-EGFR (Tyr1068) Antibody (MA5-14485) in ICC/IF Immunofluorescence analysis of EGFR was performed using 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. citizens cash back cardWebExpression of phospho-EGFR (Tyr1068, p-EGFR), phospho-ERK1/2 (Thr202/Tyr204, p-ERK1/2), phospho-AKT (Ser473, p-AKT), phospho-p53 (Ser46, p-p53), beclin 1, and LC3 in the tumor tissues was examined by Western blotting. The tissues were washed with PBS and lysed on ice in radioimmunoprecipitation assay buffer containing a complete protease ... citizens cash back plus cardWebThe GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068. A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation. Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR … dickeys libertyWebA-431 EGFR KO cells treated with EGF. Phospho-EGFR was detected at ~170 kDa using Invitrogen™ Phospho-EGFR (Tyr1068) Polyclonal Antibody (Cat. No. PA5-17848), phospho-SHC was detected at ~51 kDa using Invitrogen™ Phospho-SHC (Tyr239, Tyr240) Polyclonal Antibody (Cat. No. 44-830), phospho-MEK was detected at ~45 kDa using dickey sleeveless mock turtle neckWebThe GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR … citizens catering