WebPhiC31 integrase, a site-specific large serine recombinase, is a useful tool for genome engineering in a variety of eukaryotic species and cell types. PhiC31 integrase performs efficient recombination between its attB site and either its own placed attP site or a partially mismatched genomic pseudo attP site.

phiC31 stocks available in the Fly Facility Fly Facility

WebMar 4, 2016 · Phage integrases are prokaryotic site-specific recombinases that perform precise cut-and-paste recombination between their short attB and attP recognition sequences. These enzymes work in cellular... WebAug 11, 2011 · A generic PhiC31 integrase mediated exchange vector, pCB92, was assembled by modifying pExchange4-CB9 (derived from pRMCE [9]) with the insertion of a murine H19 insulator [20], obtained from pWHERE (Invivogen), together with a synthesized polylinker of unique sites to facilitate cloning. howard cabinet grand 26231

Paul Rowley - Associate Professor - The University of Idaho

WebOver the past decade, the integrase enzyme from phage phiC31 has proven to be a useful genome engineering tool in a wide variety of species, including mammalian cells. WebPhiC31 integrase can integrate a plasmid of any size, as a single copy, and requires no cofactors. The integrated transgenes are stably expressed and heritable. SBI’s PhiC31 … WebThe phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two … howard c171