site stats

Cstps1

WebDec 15, 2024 · However, CsTPS1 and CsTPS6 were down-regulated with the NaCl treatment and reached the lowest expression level at 24 h. Under H 2 O 2 treatment, … WebMar 6, 2015 · An enzyme encoding valencene synthase gene (Cstps1) was more abundant in Temple than in Murcott. Valencene accounted for 9.4% of total volatile content in Temple, whereas no valencene was detected in Murcott fruit. Murcott expression of Cstps1 is severely reduced. Conclusion:

Production of the sesquiterpene (+)-valencene by metabolically ...

WebOct 19, 2024 · CsTPS1-3 have predicted chloroplast-target peptides and produced predominantly linalool when GPP was supplied as substrate. Furthermore, CsTPS9 catalysed the formation of ( E )-β-ocimene and myrcene, and small amounts of sabinene and ( Z )-β-ocimene from GPP. WebMay 18, 2016 · Valencene synthesis is catalyzed by the terpene synthase CsTPS1, but the transcriptional mechanisms controlling its gene expression are unknown. Here, the AP2/ERF (APETALA2/ethylene response factor) transcription factor, CitAP2.10, is characterized as a regulator of (+)-valencene synthesis. software engineering salary in india fresher https://bwautopaint.com

Genome-wide identification and expression analysis of the …

WebBiochemical goals • In Citrus sinensis, the Cstps-1 gene codes for the production of the enzyme Citrus Valancene Synthase (CVS). • In vivo, CVS reacts with farnesyl pyrophosphate (FPP) to produce the characteristically fragrant valencene (as well as 5 … WebJan 7, 2016 · Escherichia coli (pRSF-CsTps1 and pAC-Mev/Scidi/Aacl) was similarly cultured, extracted, and analyzed by GC–MS. As a result, only one peak generated, which was determined to be linalool by the MS … WebJun 1, 2004 · The characterization of the Cstps1 gene now provides researchers with an opportunity to engineer plants for high-level production of valencene, a highly valued flavor and fragrance compound. Adapted … slower cooker pork butt

CPS1 Gene - GeneCards CPSM Protein CPSM Antibody

Category:CPS1 gene: MedlinePlus Genetics

Tags:Cstps1

Cstps1

Cloning and Characterization of a Monoterpene Synthase Gene …

WebDec 10, 2014 · The coding sequence of (+)-valencene synthase CsTPS1 ( C. sinensis) was obtained from NCBI GeneEntrez Database (GenBank: AF441124.1 ). The sequence was codon optimized for C. glutamicum by the Java Codon Adaptation Tool (JCat) ( Grote et al., 2005) and the optimized gene was synthesized by GeneArt ® life technologies™ … Web446 Planta(2024)247:443–457 13 furtherpuriedandsequenced.Theresultingsequencewas named CsTPS1 pro. Thereafter,theCsTPS1 pro sequencewasfusedtoa GUSreportergene.Forthis,368nucleotidesupstreamthe

Cstps1

Did you know?

WebCstps1 belonged to the terpene synthase-a subfamily of plant, which was the largest clade of terpene synthases and mainly composed of sesquiterpene synthases. The Cstps1 expression was detected in different organs, with high expression levels in leaf and flower, weak expression in stem and root. Real-time qRT-PCR indicated that Cstps1 WebCsTPS1 encodes for a monoterpene synthase that contributes to the emission of a blend of volatile compounds emitted from flowers of Camelina sativa. The work describes the in vitro characterization of a monoterpene synthase and its regulatory region that we cloned from Camelina sativa (Camelina). Here, we named this gene as C. sativa terpene synthase 1 …

WebDec 5, 2013 · • Citrus volatiles confer protection against endoparasitoid wasps Summary Background Egg-laying animals, such as insects, ensure the survival of their offspring by depositing their eggs in favorable environments. To identify suitable oviposition sites, insects, such as the vinegar fly Drosophila melanogaster, assess a complex range of … WebJun 1, 2004 · The corresponding residues within Cstps1 were initially identified by primary sequence alignment, then visual inspection of the relevant sequences overlaid onto the …

WebOther disorders. One common alteration (polymorphism) in the CPS1 gene has been associated with increased risk of circulatory problems in newborns and in individuals who … Webthat CitAP2.10 could trans-activate the CsTPS1 promoter. Ethylene enhanced expression of CitAP2.10 and this effect was abolished by the ethylene antagonist 1 …

WebMar 1, 2007 · Recombinant CsTPS1 showed a K m value of 6.8 μM, a V max of 1.1 × 10 −4 μmol/min and V max /K m of 0.016; the pH optimum was determined at pH 6.5, and a temperature optimum at 40°C. Recombinant CsTPS2 showed a K m value of 10.5 μM, ...

WebJul 28, 2012 · The Cstps1 expression was detected in different organs, with high expression levels in leaf and flower, weak expression in stem and root. Real-time qRT-PCR … software engineering salary in usaWebValencene synthesis is catalyzed by the terpene synthase CsTPS1, but the transcriptional mechanisms controlling its gene expression are unknown. Here, the AP2/ERF … software engineering salary singaporeWebCsTPS1- AS reduced the accumulation of geraniol, and the silenced tea plants exhibited greater susceptibility to pathogen infection than control plants. However, the geraniol content and pathogen resistance of CsTPS1-silenced plants and control plants did not signi cantly di er. Further analysis showed that silencing of CsTPS1- AS led to a ... software engineering salary los angelesWebNov 18, 2003 · Alignment of Cstps1 with tobacco and cotton sesquiterpene synthase‐deduced amino acid sequences.. Aligned sequences are of C. sinensis Cstps1 … slower corporationWebThe recombinant enzyme encoded by Cstps1 was shown to convert farnesyl diphosphate to a single sesquiterpene product identified as valencene by gas chromatography-mass spectrometry (GC-MS). Phylogenetic analysis of plant terpene synthase genes localized Cstps1 to the group of angiosperm sesquiterpene synthases. software engineering salary texasWebApr 30, 2024 · Our results uncovered seven CsTPS genes in the cucumber genome and named CsTPS1-CsTPS7 according to their locations in the chromosomes. Seven CsTPS genes were randomly distributed in six cucumber chromosomes. software engineering salary nzWebApr 30, 2024 · Our results uncovered seven CsTPS genes in the cucumber genome and named CsTPS1-CsTPS7 according to their locations in the chromosomes. Seven CsTPS genes were randomly distributed in six cucumber chromosomes. slower cooker recipe for sauerkraut and ribs